Figure 2
Hormones control metastases of luminal but not basal-like tumor cells. (A) Ovariectomized (Ovx’d) non-obese diabetic/severe combined immunodeficient gamma (NSG) mice were intracardiac-injected with 105 luciferase and ZsGreen-tagged basal-like EWD8 or luminal E3 cells and implanted with cellulose (C) or with estrogen (E) or estrogen + progestin (E+P)-releasing pellets. Representative images at necropsy of ZsG fluorescent metastases in different organs are shown. BF, bright field image (n = 20 per treatment group). (B) Bar graph shows the percentage of mice in each treatment group—C (green), E (blue), and E+P (red)—with E3 metastases to distant organs. Data are presented as mean percentages per group (n = 20 per treatment group). (C) Bar graph shows the percentage of mice in each treatment group—C (green), E (blue), and E+P (red)—with EWD8 metastases to distant organs. Data are presented as mean percentages per group (n = 20 per treatment group). (D) Number of ZsG+ metastatic sites per mouse at necropsy for E3 and EWD8 cells under C (green), E (blue), or E+P (red) conditions. Data are presented as mean ± standard error of the mean (SEM) (n = 20 per treatment group). **P <0.005, Student’s t test.