Figure 5

Transducin (β)-like 1 X-linked receptor 1 (TBLR1) contributes to breast cancer progression in vitro and in vivo . (A and B) Anchorage-independent growth assays in TBLR1-overexpressing cells and TBLR1-silenced cells, respectively. Quantification of the soft agar colony formations was based on colonies >0.1 mm in diameter after 10 days of culture. (C-E) Xenograft model in nude mice inoculated with either TBLR1-overexpressing or TBLR1-knockdown breast cancer cells, or their respective vector control cells (n =5/group) 42 days after inoculation. (C) Growth curves showing changes in tumor volume; (D) images showing changes in tumor size; (E) mean tumor weights. (F) H&E and immunohistochemical staining showed that overexpression of TBLR1 induced, whereas suppression of TBLR1 inhibited, the tumorigenicity of breast cancer cells in vivo, as indicated by the percentage of Ki-67 positive cells. All data are presented as mean ± SD of three independent experiments; *P <0.05.