Figure 5

c-Jun interacts with DDX21 in breast cancer cell lines. (A) Panel of breast cancer cell lines was screened by western blot analysis with anti-c-Jun antibody. Fifty μg of whole cell lysates were subjected to the analysis and tubulin is used as a loading control. Quantitation of the signal was made by Image J software and normalized by tubulin levels. (B) 500 μg of protein lysates were subjected to immunoprecipitation with anti-c-Jun antibody with mouse IgG as a negative control, 25 μg of SKBR3 cell lysate was used as input control. Immune complexes were blotted with anti-DDX21 and anti-c-Jun. (C) Reciprocal immunoprecipitation was also performed with the above-mentioned cell lysates. Western blot analysis was performed to confirm the association between c-Jun and DDX21 in breast cancer cell lines. (D) c-Jun immunoprecipitation was performed on purified nuclear lysates from the indicated cell lines. Western blot analysis was performed to confirm the association between c-Jun and DDX21.