Figure 3

AZA restores the expression of P-REX1 in MCF-10A and MDA-MB-231 cells. Cells were treated with 5-aza-2′-deoxycytidine (AZA) (10 μM, 96 h) and/or trichostatin A (TSA) (24 h, 100 ng/ml). Total RNA was isolated, reverse transcribed, and used for the determination of P-REX1 mRNA levels by quantitative PCR. T-47D and BT-474 breast cancer cell lines were used as positive controls for P-REX1 expression in luminal breast cancer cell lines. P-REX1 mRNA levels were normalized to the housekeeping gene B2M and expressed as relative to those in T-47D cells. Similar results were observed in two additional experiments. As P-REX1 mRNA levels in non-treated MCF-10A and MDA-MB-231 cells are non detectable (ND), statistical analysis is not provided.