Figure 4

Western blot analysis of carcinoma cell lines and tumor tissues. (A) Western blot analysis of AKT and pAKT in 202Mul and 202Mul/DN established mammary carcinoma cell lines incubated with transforming growth factor β (TGFβ) 1 ng/mL for 24 hours. (B) Western blot and quantitative analysis of AKT and pAKT in tumor tissue lysates at 5 and 12 weeks after tumor palpation. Open bars, 202Mul mice; closed bars, 202Mul/DN mice. Protein lysates for (A) and (B) were prepared through homogenization in Complete-M lysis buffer (Roche) and quantified by Bradford DC assay (Biorad). AKT and pAKT were detected using rabbit monoclonal antobody (Cell Signaling).