Figure 2

High doses of tamoxifen can skew the relative distribution of mammary cell populations over time. Absolute number of different types of mammary cells per pair of inguinal glands treated with varying doses of tamoxifen and analyzed at (A) 1 and 2 or (B) 3, 7, 21, and 56 days after tamoxifen injection (*P < 0.05; **P < 0.01; ***P < 0.0001). (C) Representative flow-cytometry dot plots show the expression of EpCAM, CD49f, Sca1, and CD49b among mammary cells from control mice and mice treated with 3 × 5 mg tamoxifen and analyzed 21 (left panels) or 56 days (right panels) later.