Figure 8
From: Breast fibroblasts modulate epithelial cell proliferation in three-dimensional in vitro co-culture
Comparative expression of insulin-like growth factor (IGF) II mRNA. IGF II expression levels in MCF10A cells, MCF10AT cells, normal breast-associated fibroblasts (NAF) and carcinoma-associated fibroblasts (CAF) were determined by quantitative real-time PCR. All expression levels are relative to the calibrator, MCF10AT cells. The error bars represent the standard deviation of triplicate assays for each sample. Comparison of the mean expression level between NAF (mean = 4.2) and CAF (mean = 7.7) did not reach statistical significance (P = 0.39, t test).