Table 3 Comparative analysis of ER by immunohistochemistry (ER1D5), DCC ([3H]oestradiol-binding) and hydroxylapatite ([3H]oestradiol-binding and EI)
From: Estrogen receptor of primary breast cancers: evidence for intracellular proteolysis
| Â | Tissular ER | Cytosolic ER | ||
|---|---|---|---|---|
| Â | IHC | DCC | Hydroxylapatite | |
Case no. | IS | n (fmol/mg protein) | n (fmol/mg protein)* | EI (%)†|
1 | 10 | 39 | 27 (20 + 7) | 74.5 |
2 | 20 | 69 | 62 (54 + 8) | 86.8 |
3 | 20 | 32 | 67 (21 + 46) | 31.5 |
4 | 40 | 38 | 35 (12 + 23) | 34.4 |
5 | 130 | 23 | 130 (91 + 39) | 70.0 |
6 | 130 | 83 | 151 (62 + 89) | 41.1 |
7 | 160 | 163 | 166 (30 + 136) | 18.0 |
8 | 160-200 | 257 | 209 (58 + 151) | 27.5 |
9 | 260 | 23 | 103 (64 + 39) | 62.1 |
10 | 360 | 265 | 230 (114 + 116) | 49.6 |
11 | 360-400 | 400 | 360 (190 + 170) | 52.6 |
12 | 360-400 | 354 | 337 (82 + 255) | 24.5 |
13 | 360-400 | 178 | 193 (60 + 133) | 31.0 |
14 | 360-400 | 92 | 174 (36 + 138) | 20.8 |
15 | 360-400 | 312 | 390 (156 + 234) | 40.0 |