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Table 3 Comparative analysis of ER by immunohistochemistry (ER1D5), DCC ([3H]oestradiol-binding) and hydroxylapatite ([3H]oestradiol-binding and EI)

From: Estrogen receptor of primary breast cancers: evidence for intracellular proteolysis

  Tissular ER Cytosolic ER
  IHC DCC Hydroxylapatite
Case no. IS n (fmol/mg protein) n (fmol/mg protein)* EI (%)
1 10 39 27 (20 + 7) 74.5
2 20 69 62 (54 + 8) 86.8
3 20 32 67 (21 + 46) 31.5
4 40 38 35 (12 + 23) 34.4
5 130 23 130 (91 + 39) 70.0
6 130 83 151 (62 + 89) 41.1
7 160 163 166 (30 + 136) 18.0
8 160-200 257 209 (58 + 151) 27.5
9 260 23 103 (64 + 39) 62.1
10 360 265 230 (114 + 116) 49.6
11 360-400 400 360 (190 + 170) 52.6
12 360-400 354 337 (82 + 255) 24.5
13 360-400 178 193 (60 + 133) 31.0
14 360-400 92 174 (36 + 138) 20.8
15 360-400 312 390 (156 + 234) 40.0
  1. *Hydoxylapatite corresponds specifically to [3H]oestradiol extracted successively from hydroxylapatite with KCl and ethanol (ie 27 = 20 + 7). EI = ([3H] oestradiol) [KCl] × 100/([3H]oestradiol) [KCl] + ([3H]oestradiol) [EtOH]. IHC, immunohistochemistry; IS, immunostaining score.