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Figure 2 | Breast Cancer Research

Figure 2

From: Investigation of three new mouse mammary tumor cell lines as models for transforming growth factor (TGF)-β and Neu pathway signaling studies: identification of a novel model for TGF-β-induced epithelial-to-mesenchymal transition

Figure 2

Evaluation of the morphologic character of the BRI-JM01, BRI-JM04, and BRI-JM05 cell lines. (a) Phase contrast microscopy revealed the typical epithelial 'cobble-stone'-like morphology for the BRI-JM01 cell line, as compared with the morphology of the BRI-JM04 and BRI-JM05 cell lines. (b) Flow cytometry revealed that the cell populations of all three cell populations are more than 95% positive for the junctional epithelial marker E-cadherin. Cells labeled with either an irrelevant primary isotype equivalent antibody (HA) or the secondary antibody alone (Alexa-488) served as negative controls (top and middle panel, respectively). (c) BRI-JM04 and BRI-JM05 cells seeded in Matrigel grew within 2 weeks, whereas BRI-JM01 cells grew at a slower rate.

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Breast Cancer Research

ISSN: 1465-542X

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