Expression of hypoxia-inducible factor-1α and cell cycle proteins in invasive breast cancer are estrogen receptor related

Table 1 Mouse monoclonal antibodies, availability, dilution, incubation, antigen retrieval and detection method used

Antibody	Company	Dilution	Incubation	Antigen retrieval	Detection
Ki-67	Dako	1:40	o/n, 4°C	MW	ABC
Cyclin A	Novocastra	1:100	o/n, 4°C	Autoclave	ABC
Cyclin D₁	Neomarkers	1:400	o/n, 4°C	Autoclave	ABC
p21	Pharmingen	1:500	o/n, 4°C	Autoclave	ABC-BT
p53	DAKO	1:50	o/n, 4°C	MW	ABC
Bcl-2	DAKO	1:50	o/n, 4°C	Autoclave	ABC-BT
ER	DAKO	1:50	o/n, 4°C	Autoclave	ABC
VEGF	R&D systems	1:40	o/n, 4°C	Autoclave	ABC
HIF-1α	Abcam	1:500	1 h, 20°C	Waterbath	CSA kit
HER-2/neu	M v/d Vijver	1:10,000	o/n, 4°C	None	ABC

Autoclave, autoclave for 20 min at 120°C; ABC, avidin-biotinyl complex; ABC-BT, biotinyl-tyramide enhancement; Mv/d Vijver, Dr M van der Vijver, Dutch Cancer Institute, Amsterdam, The Netherlands; MW, microwave in citrate buffer pH 6.0 for 10 min near to boiling; o/n, overnight; Waterbath, 45 min at 95°C.

ISSN: 1465-542X