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Table 2 Sequence analysis of isolated, hypomethylated bands

From: Chemically induced DNA hypomethylation in breast carcinoma cells detected by the amplification of intermethylated sites

Cell line/band

Band size

Location

BLAST homology (region)

Gene(s)

Details

Tissue/tumour type

MCF-7

1

395

7p14.3

RP11-162O1 (61998-61604)

None

NA

NA

2

297

2p24.1

RP11-414D15 (178657-178361)

BC013982, AL834515

mRNA (UP)

Choriocarcinoma, melanoma

3

183

9q34.3

RP11-413M3 (36274-36092)

AF032387

snRNA activating protein

Foetal cell teratocarcinoma

4

107

21q22.1

AP001713 (9409-9303)

None

NA

NA

HCC1806

5

481

9q31.2

RP11-438P9 (61700-61218)

MIRb

SINE repeat

NA

6

297

2p24.1

RP11-414D15 (178657-178361)

BC013982, AL834515

mRNA (UP)

Choriocarcinoma, melanoma

7

197

11q13.3

RP11-211G23 (138454-138261)

None

NA

NA

MDA-MB 231

8

183

9q34.3

RP11-413M3 (36274-36092)

AF032387

snRNA activating protein

Foetal cell teratocarcinoma

9

156

11q13

CTD-3074O7 (121538-121383)

M86407, AK125851

α-actinin 3

Foetal skeletal muscle

10

122

10q26

RP13-137A17 (59898-60011)

None

NA

NA

MDA-MB 468

11

183

9q34.3

RP11-413M3 (36274-36092)

AF032387

snRNA activating protein

Foetal cell teratocarcinoma

12

156

11q13

CTD-3074O7 (121538-121383)

M86407, AK125851

α-actinin 3

Foetal skeletal muscle

  1. Band size, location, and BLAST homology were determined using BLAST [40]. Genes, details and expressing tissue were determined using human genome browser software [41]. 'Tissue/tumour type' refers to the particular cell type or carcinoma in which these mRNAs were previously shown to be expressed. UP, unknown protein.