Chemically induced DNA hypomethylation in breast carcinoma cells detected by the amplification of intermethylated sites

Table 1 5-Azacytidine induced hypomethylation analysis in the breast carcinoma cells

Cell line	Time point	Number of intense bands	% Hypomethylation
MCF-7	48 hours	41	43.90
	72 hours	41	43.90
	96 hours	41	53.65
HCC1806	48 hours	23	0
	72 hours	23	0
	96 hours	22	36.36
MDA-MB 231	48 hours	29	0
	72 hours	29	0
	96 hours	26	11.53
MDA-MB 468	48 hours	24	0
	72 hours	24	0
	96 hours	17	23.52

The number of intense bands as observed is indicated for the nontreated lanes of each cell line from Fig. 1 at each time point. The percentage hypomethylation for each cell line at each time point is shown as percentage loss of bands compared with the corresponding nontreated lane.

ISSN: 1465-542X