Figure 1
From: The Icelandic founder mutation BRCA2 999del5: analysis of expression
Overexpression of BRCA2 999del5 mRNA in COS7 cells. The potential coding sequence of BRCA2 999del5 was cloned into the pIND(SP1)/V5-His expression vector, transfected into COS7 cells and induced with ponasterone A: 1, mock transfection; 2, cells transfected with pIND-BRCA2 999del5; and 3, untransfected COS7 cells. (a) Expression of BRCA2 999del5 was confirmed by northern blotting with a labeled BRCA2 999del5 probe. (b) RNA samples that were used in northern blotting stained with ethidium bromide and visualized with UV light.