Figure 6

Involution related expression profiles: apoptosis and immune related genes. (a) Expression profiles of differentially expressed immune cell antigens, illustrating the relative proportions of immune cell subtypes in mammary gland during the complete pregnancy cycle. Monocyte/macrophage markers: Lrp1 = Cd91; Csf1r = Cd115. Lymphocyte markers: Tnfrsf9 = Cd137; H2-M1 = Mb1; Tnfrsf4 = OX40. (b) Profiles of differentially expressed anti-inflammatory factors in the complete mammary time course. (c) Profiles of differentially expressed soluble defence factors, including the innate components lactoferrin (Ltf), Cd14, Ly86 and Lbp. Also shown is the mean expression profile for all differentially expressed immunoglobulin transcripts. (d) Differentially expressed death receptor ligands (Tnfsf6 = FasL, Tnfsf12 = Tweak, TNFa and Tnfsf10 = Trail) and the insulin-like growth factor (IGF) inhibitor Igfbp5. (e) Differentially expressed receptors and ligands involved in recognition and engulfment of apoptotic cells. Y axes indicate the normalized intensity. (Note that the brackets in panel d represent scale for Igfbp5 only.) X axes indicate developmental time points for the complete 12-point time course (panels a, b and c) and 7-point lactation/involution time course (d, e).