Figure 4
From: A novel cell culture model for studying differentiation and apoptosis in the mouse mammary gland
Effects of growth temperature on morphology and anchorage independent growth of KIM-2. (a) Cells isolated and grown at 37°C showing the typical cuboidal epithelial morphology. (b) Cells isolated and grown at 33°C have a spindle-like mesenchymal morphology. Scale bar for (a) and (b) 50 μm. (c) Cells, isolated and grown at 37°C, seeded in soft agar and cultured for 12 days. Cells do not form colonies. (d) Cells, isolated and grown at 33°C, seeded in soft agar and cultured for 12 days at 37°C showing the formation of colonies. Scale bar for (c) and (d) 10 μm.