Figure 1
From: A novel cell culture model for studying differentiation and apoptosis in the mouse mammary gland
BLG-tsA58 transgenic mice. (a) Scheme of vector construct; 4.2 kb of the ovine BLG promoter including the noncoding exon 1 was fused to the 'enhancerless' SV40 T-Ag temperature sensitive mutant tsA58. The restriction sites shown are Sall (S), BamHI (B), Stul (St), and Ndel (N). The EcoRV and Bgll sites were destroyed during the construction of this vector. (b) The transgene copy number in the five surviving transgenic lines was estimated by Southern blotting. Digested genomic liver DNA (10 μg) from three sisters in each of the lines was analyzed with sheep genomic liver DNA used as a single copy control. The blot was hybridized with probe 1, and stripped and rehybridized with a WAP probe as a loading control.