Figure 1
From: Id-1 is not expressed in the luminal epithelial cells of mammary glands
Immunoblot analyses for Id-1 in various mouse tissues. Lysates were prepared from various mouse tissues and analyzed for Id-1, using C-20 antibody, as described in the text. The positions of the molecular weight standards (kDa) are shown on the right. Lanes 1 and 3, wild type mammary glands (129Sv/C57BL and FVB, respectively); lane 2, mammary glands from Id-1 null mutant mouse; lanes 4–6, testis, uterus and intestine from wild type mice, respectively; lane 7, mammary gland extract of wild type mice in which treatment with the primary antibody was performed in the presence of the blocking peptide.