Table 1 Effect of persistent organochlorines on transient BRCA1-luciferase (pGL12) reporter gene expression in MCF-7(BUS) and MDA-MB-231

Control

18 ± 2.9

100 ± 11.8

18 ± 0.3

100 ± 6.3

18 ± 0.8

100 ± 8.4

10 μM

36 ± 8.3^*

59 ± 8.6^*

5 ± 1.1^*

8 ± 3.4^*

n.d.

n.d.

1 μM

20 ± 2.5

96 ± 25.2

7 ± 0.5^*

7 ± 0.6^*

n.d.

n.d.

100 nM

20 ± 6.8

102 ± 16.8

5 ± 0.4^*

5 ± 1.7^*

7 ± 0.9^*

46 ± 4.6^*

10 nM

24 ± 8.1

95 ± 7.4

5 ± 0.7^*

8 ± 2.4^*

7 ± 0.8^*

33 ± 2.6^*

1 nM

23 ± 8.1

88 ± 21.8

5 ± 0.7^*

46 ± 10.8^*

8 ± 1.3^*

39 ± 3.5^*

100 pM

24 ± 9.3

108 ± 11.4

9 ± 0.3^*

81 ± 5.0^*

14 ± 2.5^*

66 ± 12.6^*

10 pM

17 ± 1.7

112 ± 4.2

12 ± 0.8^*

109 ± 25.1

16 ± 4.0

110 ± 24.1

1 pM

21 ± 3.4

92 ± 18.9

15 ± 2.3

101 ± 55.8

25 ± 6.7

107 ± 13.8

MCF-7(BUS)

Control

18 ± 3.0

100 ± 7.8

18 ± 2.1

100 ± 6.9

18 ± 1.7

100 ± 12.7

10 μM

7 ± 1.2^*

59 ± 14.9^*

8 ± 0.7^*

75 ± 9.0^*

9 ± 3.5^*

70 ± 11.5^*

1 μM

17 ± 1.5

81 ± 19.5

16 ± 3.5

92 ± 17.2

13 ± 4.0

105 ± 11.7

100 nM

16 ± 1.7

90 ± 4.9^*

21 ± 0.9

97 ± 12.9

16 ± 1.4

110 ± 24.7

10 nM

17 ± 3.9

82 ± 10.5^*

22 ± 4.4

113 ± 10.6

19 ± 5.4

107 ± 28.9

1 nM

19 ± 3.0

87 ± 4.0^*

20 ± 5.1

131 ± 28.9

23 ± 7.9

94 ± 10.2

100 pM

18 ± 4.1

88 ± 6.5^*

21 ± 6.4

95 ± 16.5

17 ± 6.4

108 ± 31.1

10 pM

19 ± 2.2

85 ± 21.2

22 ± 0.9

112 ± 26.7

21 ± 7.4

112 ± 11.3

1 pM

24 ± 5.3

86 ± 20.3

19 ± 6.2

111 ± 9.5

16 ± 5.0

109 ± 21.2

MDA-MB-231

pGL12

93 ± 19.3

21 ± 8.1^*

65 ± 11.5^*

35 ± 1.3^*

50 ± 10.6^*

49 ± 7.0^*

pGL12 + 10 nM E2

100 ± 15.0

21 ± 6.6^*

69 ± 8.6^*

32 ± 6.9^*

53 ± 6.0^*

44 ± 5.6^*

pGL12/ER

57 ± 10.4

35 ± 11.6^*

55 ± 15.5

45 ± 10.6

46 ± 12.8

48 ± 13.5

pGL12/ER + 10 nM E2

100 ± 13.8

37 ± 21.3^*

74 ± 35.8

65 ± 23.9^*

60 ± 24.5^*

58 ± 36.5