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Table 1 Effect of persistent organochlorines on transient BRCA1-luciferase (pGL12) reporter gene expression in MCF-7(BUS) and MDA-MB-231

From: Inhibition of E2-induced expression of BRCA1 by persistent organochlorines

MCF-7(BUS) Tox.Tech. Tox.Tech. +100 pM E2 ICI 182780 ICI 182780 +100 pM E2 TCDD TCDD +100 pM E2
Control 18 ± 2.9 100 ± 11.8 18 ± 0.3 100 ± 6.3 18 ± 0.8 100 ± 8.4
10 μM 36 ± 8.3* 59 ± 8.6* 5 ± 1.1* 8 ± 3.4* n.d. n.d.
1 μM 20 ± 2.5 96 ± 25.2 7 ± 0.5* 7 ± 0.6* n.d. n.d.
100 nM 20 ± 6.8 102 ± 16.8 5 ± 0.4* 5 ± 1.7* 7 ± 0.9* 46 ± 4.6*
10 nM 24 ± 8.1 95 ± 7.4 5 ± 0.7* 8 ± 2.4* 7 ± 0.8* 33 ± 2.6*
1 nM 23 ± 8.1 88 ± 21.8 5 ± 0.7* 46 ± 10.8* 8 ± 1.3* 39 ± 3.5*
100 pM 24 ± 9.3 108 ± 11.4 9 ± 0.3* 81 ± 5.0* 14 ± 2.5* 66 ± 12.6*
10 pM 17 ± 1.7 112 ± 4.2 12 ± 0.8* 109 ± 25.1 16 ± 4.0 110 ± 24.1
1 pM 21 ± 3.4 92 ± 18.9 15 ± 2.3 101 ± 55.8 25 ± 6.7 107 ± 13.8
MCF-7(BUS) PCB#138 PCB#138 +100 pM E2 PCB#153 PCB#153 +100 pM E2 PCB#180 PCB#180 +100 pM E2
Control 18 ± 3.0 100 ± 7.8 18 ± 2.1 100 ± 6.9 18 ± 1.7 100 ± 12.7
10 μM 7 ± 1.2* 59 ± 14.9* 8 ± 0.7* 75 ± 9.0* 9 ± 3.5* 70 ± 11.5*
1 μM 17 ± 1.5 81 ± 19.5 16 ± 3.5 92 ± 17.2 13 ± 4.0 105 ± 11.7
100 nM 16 ± 1.7 90 ± 4.9* 21 ± 0.9 97 ± 12.9 16 ± 1.4 110 ± 24.7
10 nM 17 ± 3.9 82 ± 10.5* 22 ± 4.4 113 ± 10.6 19 ± 5.4 107 ± 28.9
1 nM 19 ± 3.0 87 ± 4.0* 20 ± 5.1 131 ± 28.9 23 ± 7.9 94 ± 10.2
100 pM 18 ± 4.1 88 ± 6.5* 21 ± 6.4 95 ± 16.5 17 ± 6.4 108 ± 31.1
10 pM 19 ± 2.2 85 ± 21.2 22 ± 0.9 112 ± 26.7 21 ± 7.4 112 ± 11.3
1 pM 24 ± 5.3 86 ± 20.3 19 ± 6.2 111 ± 9.5 16 ± 5.0 109 ± 21.2
MDA-MB-231 Control Tox.Tech. (10 μM) TCDD(10 nM) PCB#138 (10 μM) PCB#153 (10 μM) PCB#180 (10 μM)
pGL12 93 ± 19.3 21 ± 8.1* 65 ± 11.5* 35 ± 1.3* 50 ± 10.6* 49 ± 7.0*
pGL12 + 10 nM E2 100 ± 15.0 21 ± 6.6* 69 ± 8.6* 32 ± 6.9* 53 ± 6.0* 44 ± 5.6*
pGL12/ER 57 ± 10.4 35 ± 11.6* 55 ± 15.5 45 ± 10.6 46 ± 12.8 48 ± 13.5
pGL12/ER + 10 nM E2 100 ± 13.8 37 ± 21.3* 74 ± 35.8 65 ± 23.9* 60 ± 24.5* 58 ± 36.5
  1. All values are corrected with β-galactosidase values (MCF-7(BUS)) or protein (MDA-MB-231) and then normalized to 17β-estradiol (E2)-induced cells (set to 100). The data are mean ± standard deviations of at least five or six measurements from two independent experiments. pGL12/ER, cotransfection with the pHEO1-expressing estrogen receptor alpha; Tox.Tech., toxaphene (technical mixture); TCDD, 2,3,7,8 tetrachlorodibenzo-p-dioxin; PCB, polychlorinated biphenyl. * P < 0.05 relative to the given control (ethanol/DMSO or E2).