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Figure 5 | Breast Cancer Research

Figure 5

From: Peroxiredoxin-1 protects estrogen receptor α from oxidative stress-induced suppression and is a protein biomarker of favorable prognosis in breast cancer

Figure 5

Effect of PRDX1 knockdown and overexpression on the response of ERα protein expression following induction of oxidative stress in breast cancer cells. (A) PRDX1 knockdown sensitizes these ER-positive cells to H2O2-mediated ERα protein suppression while overexpression allows the cells resistance to this suppression. (B) PRDX1 knockdown sensitizes these cells to the concurrent phosphorylation of Akt at Serine-473 after treatment with increasing concentrations of H2O2, while it does not markedly alter expression of E-cadherin or phosphorylation of ERK1/2 after treatment with increasing concentrations of H2O2 for 16 hours. (C) PRDX1 knockdown sensitizes the cells to peroxynitrite-induced suppression of ERα and phosphorylation of Akt. (D) Inhibition of the antioxidant activity of PRDX1 using adenanthin suppresses ERα levels in ZR-75-1 and T47D cells, with gradual PRDX1 monomer formation. All treatments were 16 hours in duration. (Comp: complete medium only; DegPer: degraded peroxynitrite). ER, estrogen receptor; H2O2, hydrogen peroxide; PRDX1, peroxiredoxin 1.

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Breast Cancer Research

ISSN: 1465-542X

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