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Figure 2 | Breast Cancer Research

Figure 2

From: Aldehyde dehydrogenase and estrogen receptor define a hierarchy of cellular differentiation in the normal human mammary epithelium

Figure 2

ALDE+/ER– cells generate ER+ cells in vitro and in vivo . (A-F) Ducts generated by the ALDE+ mammary epithelial cells injected in the humanized cleared fat pad of NOD/scid mice: (A, B) Fat-pad section stained with H&E. (C) Low-magnification image showing section through epithelial duct and emerging branching ductules stained for SMA (AEC, red). (D-F) Ductule section stained for myoepithelial marker SMA (D, AEC, red), luminal marker CK18 (E, DAB, brown), and ER (F, DAB, nuclear brown staining). (G,H) Mammospheres generated by ALDE+ cells contained ER+ and PR+ cells (DAB, nuclear brown). Double staining for ER and ALDH1A1 showed no overlap between ALDH1A1+ cells (AEC, cytoplasmic red, arrowhead) and the ER+ cells (arrows). Scale bar = 50 μm.

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Breast Cancer Research

ISSN: 1465-542X

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