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Table 1 Nuclear algorithm settings for the DIA calibration after the Genie classifier

From: A methodology to ensure and improve accuracy of Ki67 labelling index estimation by automated digital image analysis in breast cancer tissue

Algorithm setting DIA-0 DIA-1 DIA-2
Averaging radius (μ) 1 1 1
Curvature threshold 2.5 2.5 2.5
Segmentation type Cytoplasm rejection Cytoplasm rejection Cytoplasm rejection
Threshold type Edge threshold Edge threshold Edge threshold
Lower intensity threshold 0 0 0
Upper intensity threshold 220 230 230
Min. nuclear size (μ2) 20 45 40
Max. nuclear size (μ2) 1,000,000 1,000 1,000
Min, roundness 0.1 0.1 0.1
Min. compactness 0 0 0.2
Min. elongation 0.1 0.1 0.2
Remove light objects removes no nuclei removes no nuclei removes no nuclei
Weak (1+) threshold 210 210 229
Moderate (2+) threshold 188 188 188
Strong (3+) threshold 162 162 162
Black threshold 0 0 0
Edge trimming Weighted Weighted Weighted
  1. DIA-0 (default), DIA-1 (subjective) and DIA-2 (based on quantified bias). Modified settings are highlighted in bold.