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Table 2 Engraftment capacity of different cell populations sorted from cryopreserved tumor cells

From: Malignant phyllodes tumors display mesenchymal stem cell features and aldehyde dehydrogenase/disialoganglioside identify their tumor stem cells

BC-P007 4 × 104 2 × 104 1 × 104 5 × 103 4 × 103 1 × 103 500 100 50 20 Frequency Distribution#
ALDH+   3/8    3/8 1/3 1/7     1:2.0 × 104 75%
ALDH-   0/4    0/6 1/7 0/3 0/4    1:1.1 × 105 25%
ALDH+/GD2+   2/2    3/3 6/10   5/10 2/20 0/3 1:6.1 × 102  
ALDH-/GD2- 0/4   0/1 0/8 2/10 0/15      1:1.3 × 105  
ALDH+   3/8    3/8 1/3 1/7     1:2.0 × 104  
ALDH+/GD2+   2/2    3/3 6/10   5/10 2/20 0/3 1:6.1 × 102  
BC-P515 1 × 10 5 2 × 10 4 1 × 10 4   4 × 10 3 1 × 10 3 500 100   50 Frequency Distribution #
ALDH+   1/4 0/3   1/4 3/9   1/5    1:2.0 × 104 96%
ALDH-   0/4 0/3   0/2 0/9      1:>4.2 × 105 4%
ALDH+/GD2+   1/3* 7/10    4/5 0/1 2/7   1/2 1:7.1 × 103  
ALDH-/GD2- 0/1 0/2 1/9   0/4 0/10      1:2.5 × 105  
ALDH+   1/4 0/3   1/4 3/9   1/5    1:2.0 × 104  
ALDH+/GD2+   1/3 7/10    4/5 0/1 2/7   1/2 1:7.1 × 103  
  1. BC-P007 and BC-P515 cryopreserved tumor cells from patient-derived xenografts of PT were sorted by FACS into ALDEFLUOR-positive, ALDEFLUOR-negative or ALDEFLUOR and GD2 double positive and double negative cells. Varying numbers of the sorted cells were injected in fat pads of NOD-SDID mice to evaluate their tumor-initiating potential. The frequency of cancer-initiating cells was analyzed using ELDA software.
  2. *:Including one of three mice died the day after tumor injection.
  3. #:The distribution of tumor-initiating cells among the BC-P007 and BC-P515 ALDH + and ALDH- cells.
  4. :Frequency was calculated by ELDA software, although the P-value for likelihood ratio test of single-hit model in each group was less than P = 0.5.