RIP2 knockdown does not cause decrease in proliferation but does cause decreased tumor growth and lung metastasis in vivo with docetaxel treatments. (A) Western blot showing relative RIP2 expression in sh-control and sh-RIP2 MDA-MB-231 cells. (B) Relative proliferation rate of sh-control and sh-RIP2 MDA-MB-231 cells in vitro. (C) Tumor volume at various time points after xenograft placements. Images are representative of final tumors at 10 weeks for sh-control (top) and sh-RIP2 (bottom). Statistics per Student t-test at final time point recorded. Representative H&E stains of lung sections at 10 weeks are shown (D and E). Scale bar represents 100 μm. Representative RIP2 fluorescence in situ hybridization (FISH) images of lung sections are shown in (F) and (G). Red, RIP2 mRNA signal. Blue, 4',6-diamidino-2-phenylindole (DAPI) nuclear stain. Scale bar represents 25 μm.