Figure 1

NDRG2 is correlated with increased survival and negatively correlated with GLUT1 in breast carcinoma. Kaplan–Meier analysis was carried out according to N-myc downstream-regulated gene 2 (NDRG2) expression levels of disease-free survival (A) and overall survival (B). (C) Serial immunostained sections for NDRG2 and glucose transporter 1 (GLUT1) in breast cancer and normal tissues were analysed. Original magnification, 40× (top) and 400× (bottom); scale bars = 50 μm. (D) Protein was extracted from matched breast tumour tissue (T) and adjacent normal tissue (N) and subjected to immunoblot analysis to examine NDRG2 and GLUT1 expression. β-actin served as a loading control. P: patient. Relative expression levels of NDRG2 (E) and GLUT1 (F) in human breast cancer and adjacent normal tissue are shown. immunoreactivity score distribution of cancer and adjacent normal tissue were represented with black and brown closed circles, respectively. The horizontal lines presented are means; error bars represented SD from 30 samples. P < 0.01 was considered a statistically significant difference.