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Table 4 Validation cohort

From: Next-generation transcriptome sequencing of the premenopausal breast epithelium using specimens from a normal human breast tissue bank

Gene logFC (RNA-Seq) Pvalue Fold change (qPCR biological validation) Validation status
AURKB 1.694476904 1.10E-05 8.56 V
BRCA1 1.042815222 4.95E-06 2.39 V
BUB1 1.526455024 2.82E-07 2.95 V
BUB1B 1.602146974 8.86E-07 3.87 V
CCNB1 1.055253675 9.55E-05 2.73 V
CDC25C 2.501848634 4.13E-11 6.1 V
CDC6 1.49654134 2.33E-08 3.76 V
CDK1 1.965582801 6.77E-12 6.33 V
CENPE 1.462857192 1.39E-06 3.05 V
CLSPN 1.383625655 6.57E-06 6.07 V
E2F1 0.996887203 0.000372 3.06 V
ECT2 0.872573718 0.000152 1.89 V
ERCC6L 1.102811499 8.88E-05 0.79 N
FANCA 1.224811173 1.63E-06 4.33 V
FOXM1 1.92032584 2.17E-07 8.43 V
HIST1H2AH 1.485622497 0.000196 0.99 N
HIST1H2AJ 2.284502132 8.84E-06 2.96 V
HIST1H2AM 1.530373984 0.000151 1.98 V
HIST1H2BH 1.563333889 3.04E-07 1.32 V
HIST1H3B 2.979880506 6.94E-16 1.43 V
KIF20A 1.876073752 5.20E-08 8.09 V
KIF23 1.604227334 1.98E-09 3.22 V
NCAPG 1.888216879 2.37E-10 7.2 V
PCNA 0.939041116 1.44E-05 2.09 V
RAD51 1.255859843 1.79E-05 4.75 V
RRM2 2.285244825 5.90E-09 1.3 V
TK1 1.410412144 2.83E-05 5.67 V
TPX2 2.025558609 1.21E-10 5.49 V
TYMS 1.667388087 2.36E-09 5.64 V
  1. TaqMan qPCR was performed for 29 genes representing the DNA replication and mitosis clusters. Twenty-seven of 29 were validated. RNA-Seq, whole transcriptome sequencing; V, validated; N, not validated.