Next-generation transcriptome sequencing of the premenopausal breast epithelium using specimens from a normal human breast tissue bank

Table 4 Validation cohort

Gene	logFC (RNA-Seq)	Pvalue	Fold change (qPCR biological validation)	Validation status
AURKB	1.694476904	1.10E-05	8.56	V
BRCA1	1.042815222	4.95E-06	2.39	V
BUB1	1.526455024	2.82E-07	2.95	V
BUB1B	1.602146974	8.86E-07	3.87	V
CCNB1	1.055253675	9.55E-05	2.73	V
CDC25C	2.501848634	4.13E-11	6.1	V
CDC6	1.49654134	2.33E-08	3.76	V
CDK1	1.965582801	6.77E-12	6.33	V
CENPE	1.462857192	1.39E-06	3.05	V
CLSPN	1.383625655	6.57E-06	6.07	V
E2F1	0.996887203	0.000372	3.06	V
ECT2	0.872573718	0.000152	1.89	V
ERCC6L	1.102811499	8.88E-05	0.79	N
FANCA	1.224811173	1.63E-06	4.33	V
FOXM1	1.92032584	2.17E-07	8.43	V
HIST1H2AH	1.485622497	0.000196	0.99	N
HIST1H2AJ	2.284502132	8.84E-06	2.96	V
HIST1H2AM	1.530373984	0.000151	1.98	V
HIST1H2BH	1.563333889	3.04E-07	1.32	V
HIST1H3B	2.979880506	6.94E-16	1.43	V
KIF20A	1.876073752	5.20E-08	8.09	V
KIF23	1.604227334	1.98E-09	3.22	V
NCAPG	1.888216879	2.37E-10	7.2	V
PCNA	0.939041116	1.44E-05	2.09	V
RAD51	1.255859843	1.79E-05	4.75	V
RRM2	2.285244825	5.90E-09	1.3	V
TK1	1.410412144	2.83E-05	5.67	V
TPX2	2.025558609	1.21E-10	5.49	V
TYMS	1.667388087	2.36E-09	5.64	V

TaqMan qPCR was performed for 29 genes representing the DNA replication and mitosis clusters. Twenty-seven of 29 were validated. RNA-Seq, whole transcriptome sequencing; V, validated; N, not validated.

ISSN: 1465-542X