Figure 1

Hierarchic clustering of the downstream-activated PI3K and/or MAPK proteins. Heat map representing unsupervised hierarchic clustering of tumor samples and corresponding downstream activated proteins in the PI3K and/or MAPK pathways from patients for whom the status of all five proteins was known (N = 350). Patients are represented horizontally. Phosphorylated proteins are indicated vertically. Red represents high/any expression of phosphorylated protein, and green represents no/low expression of phosphorylated protein (dichotomization was performed according to the Akaike information criteria [29]). In addition, the presence (red) or absence (green) of different molecular alterations in the PI3K and or MAPK pathways is shown. ⁽¹⁾Defined as the presence of either a PIK3CA mutation, positive HER2 status, or IGF-1R overexpression (IHC score 3).