Figure 2

Time course of AZD8055 effect on mTORC1 and TORC2 signalling pathways in TamR and MCF7-X cells. Western blots of 70% confluent TamR (A) and MCF7-X (B) cells treated for 15 minutes to 24 hours with AZD8055 (0 to 100 nM). Blots were probed with phospho- and total antibodies for mTORC1 and two signalling pathways. In both cell lines mTORC1 and mTORC2 signalling pathways were rapidly inhibited by AZD8055. Blots shown are representative of at least two independent experiments. Western blots of 70% confluent TamR and MCF7-X cells treated for 15 minutes to 48 hours with AZD8055 (0 to 100 nM) probed with antibodies for MAPK activity (p-erk1/2) showing no effect (C). Blots shown are from representative experiments.