Direct repression of MYB by ZEB1 suppresses proliferation and epithelial gene expression during epithelial-to-mesenchymal transition of breast cancer cells

Table 2 Antibodies used for IF, ICC, and IHC

Primary antibody	Species	Source
Snail1	Rat	Cell Signaling Technology, Beverly, MA, USA (cat. no. 4719)
Snail2	Rabbit	Abcam, Cambridge, UK (cat. no. ab27568)
Zeb1	Goat	Santa Cruz Biotechnology, Santa Cruz, CA, USA (cat. no. sc-10572)
Vimentin	Mouse	DAKO, Campbellfield, VIC (cat. no. H 7095)
E-cadherin	Rabbit	Abcam, Cambridge, UK (cat. no. ab40772)
E-cadherin	Mouse	BDT (cat. no. 612130)
MYB	Mouse	Millipore, Billerica, MA, USA (cat. no. 05–175)
α-pan actin	Mouse	Abcam, Cambridge, UK (cat. no. ab75373)
ER-α	Mouse	DAKO, Campbellfield, VIC (cat. no. M 7047)
Cytokeratin	Mouse	DAKO, Campbellfield, VIC (cat. no. M 0821)
CT3 MYB w CT5	Mouse	Synthesized in lab of Prof. Gonda
Secondary antibody	Species	Source
Anti-mouse Alexa 488	Goat	Invitrogen, Camarillo, CA, USA (cat. no. A-11011)
Anti-mouse biotin	Rabbit	DAKO, Campbellfield, VIC (cat. no. E0354)
Anti-goat biotin	Rabbit	Vector Laboratories, Burlingame, CA, USA (cat. no. BA-5000)
Anti-rabbit biotin	Swine	DAKO, Campbellfield, VIC (cat. no. E0431)
Tertiary label		Source
Streptavidin/HRP		DAKO, Campbellfield, VIC (cat. no. P0397)
DAPI		(4′,6-Diam idino-2-phenylindole, dihydrochloride)

ISSN: 1465-542X