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Table 1 mRNA expression of MCF7 cells after exposure to ASCs

From: Obesity associated alterations in the biology of adipose stem cells mediate enhanced tumorigenesis by estrogen dependent pathways

Functional gene grouping

Gene

MCF7 cells gene expression after CC with

Ob-Ab-

Ob-Ab+

Ob+Ab-

Ob+Ab+

Cell cycle

CDKN2A

1.41

1.20

2.40

50.33*

 

CCND2

1.41

1.27

2.40

1.77*

 

PTEN

1.05

1.04

−1.07

−1.18*

Apoptosis

GSTP1

9.79

22.15

17.99

2,475.21*,#

 

BAD

2.21*

2.01*

1.85*

1.33*

 

SFRP1

1.40

1.23

2.45

56.75#

Angiogenesis

SERPINE1

15.72*

33.81*

27.27*

4,672.57*,#

 

IL6

3.47*

5.44*

7.01*

197.63*,#

 

CDH13

3.65*

4.02*

4.64*

24.70*,#

 

PLAU

1.99

2.12

3.40

47.07#

 

SLIT2

3.15

2.42

1.05

30.48#

Signal transduction

     

 Hedgehog

SNAI2

4.47*

11.26*

7.41*

1,592.05*,#

 Notch

BIRC5

3.17*

3.36*

3.42*

1.84*,#

 Steroid receptor-mediated

PGR

4.15*

5.84*

12.72*

75.41*,#

 Steroid receptor-mediated

ESR1

3.97*

4.68*

16.87*

26.85*,#

Glucocorticoid

IGFBP3

15.47*

32.51*

15.03*

2,746.41*,#

 

NME1

2.86*

3.03*

3.12*

2.02*

Metastasis

MMP2

12.22*

24.35*

11.05*

2,320.15*,#

 

MMP9

3.52*

3.59*

4.46*

1.77*,#

 

TWIST1

1.45

1.31

2.51

278.20#

 

PTGS2

1.34

1.15

2.30

81.38#

Adhesion

THBS1

1.05

1.31

1.16

54.82*

 

CSF1

1.99

2.12

1.01

42.71*

 

ADAM23

1.42

1.33

1.27

7.50*

Xenobiotic transport

ABCG2

12.56*

12.09*

29.09*,#

12.70*

  1. Up-regulated (+) or down-regulated (−) gene expression is shown as fold change relative to MCF7 cells without previous co-culture with ASCs. Values are normalized to MCF7 cells without exposure to ASCs.
  2. *P <0.05; #P <0.01.