Figure 2

Changes in the expression of cell cycle regulators and steroid receptors of MCF7 cells. MCF7 cells were grown alone or in the presence of ASCs (n = 6 donors per group) grouped by the donor’s obesity status and depot site of ASCs for seven days. Cell lysate from sorted GFP⁺ MCF7 cells was collected for Western blot analysis of CDKN2A, GSTP1, SFRP1, ESR1 and PGR. Blots were then stripped and probed for actin as a control.