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Figure 1 | Breast Cancer Research

Figure 1

From: Obesity associated alterations in the biology of adipose stem cells mediate enhanced tumorigenesis by estrogen dependent pathways

Figure 1

Direct co-culture of breast cancer cells with ASCs result in increased proliferation in vitro . (A) After seven days of co-culture (CC) with six adipose stem cells (ASC) donors per group, quantification of MCF7 cells and MDA-MB-231 (231) cells was based on the percentage of GFP+ cells in the population multiplied by the total number of cells in each condition. (B) To determine the influence of ASCs on MCF7 cells indirectly, conditioned media (CM) from pooled ASCs (n = 6 donors per group) were collected and added to MCF7 cells. After seven days, the total number of MCF7 cells was counted. Values reported are the mean of three independent experiments, each performed in triplicate. Bars, ± SD. *, P <0.05; #, P <0.01.

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