Figure 6

The combination of MM-121 and paclitaxel significantly inhibits tumor cell proliferation, reduces expression of Survivin, and induces apoptosis in an in vivo mouse model. The tumors obtained from the animal studies described above were evaluated by immunohistochemical (IHC) analysis of Ki-67, Survivin, and cleaved caspase-3. (A) Data show the representative images of the immunostainging of Ki-67, Survivin, and cleaved caspase-3. (B) The IHC slides were observed by two independent personnel. The tumor cells with positive staining of Ki-67, Survivin, or cleaved caspase-3 were counted from three randomly selected areas in each slide. The three areas were first identified by scanning the entire slide at × 100 magnification, and then the positive-stained cells were counted at × 200 magnification using an Olympus BX40 microscope. The bar graphs show the average of positive staining cells in each field. Bars represent SD. ^*P <0.002 versus control or MM-121. ^**P <0.001 versus control or single-agent treatment.