Figure 2
Knockdown of SR-BI attenuates HDL 3 -induced signal transduction in MDA-MB-231 and MCF7 cells. (A,B) MDA-MB-231 and MCF7 cells were stably transduced by lentiviruses carrying either shRNA against SR-BI (shSRBI) or control shRNA (shCTL). Cells were incubated with either 10% FBS for 30 minutes or with 100 μg/ml HDL3 for the indicated times. Whole-cell lysates were analyzed by Western blot for the indicated proteins. The arrow indicates the protein corresponding to phospho-Akt. GAPDH was used as loading control. (C,D) MDA-MB-231 and MCF7 cells were stably transduced by lentiviruses carrying either shRNA against SR-BI (shSRBI) or control shRNA (shCTL). Cells were incubated with either 10% FBS for 30 minutes or with 100 μg/ml LDL for the indicated times. Whole-cell lysates were analyzed by Western blot for the indicated proteins. The arrow indicates the protein corresponding to phospho-Akt. GAPDH was used as loading control. Note that, in panel C, the image was spliced (between lines 5 and 6) to eliminate an irrelevant line.