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Table 2 Source, clone and dilution of antibodies used in this study a

From: Metabolic interaction between cancer cells and stromal cells according to breast cancer molecular subtype

Antibody Clone Dilution Company
Molecular subtype-related    
 ER SP1 1:100 Thermo Scientific, Waltham, MA, USA
 PR PgR 1:50 Dako Denmark AS, Glostrup, Denmark
 HER2 Polyclonal 1:1,500 Dako Denmark AS, Glostrup, Denmark
 Ki-67 MIB-1 1:150 Dako Denmark AS, Glostrup, Denmark
Glycolysis-related    
 Glut-1 SPM498 1:200 Abcam, Cambridge, UK
 CAIX Polyclonal 1:100 Abcam, Cambridge, UK
 MCT4 Polyclonal 1:100 Santa Cruz Biotechnology, Santa Cruz, CA, USA
Mitochondrial status-related    
 BNIP3 Ana40 1:100 Abcam, Cambridge, UK
Mitochondrial metabolism-related    
 ATP synthase 15H4C4 1:100 Abcam, Cambridge, UK
 Glutaminase Polyclonal 1:100 Abcam, Cambridge, UK
Autophagy-related    
 Beclin-1 Polyclonal 1:100 Abcam, Cambridge, UK
 LC3A EP1528Y 1:100 Abcam, Cambridge, UK
 LC3B Polyclonal 1:100 Abcam, Cambridge, UK
 p62 SQSTM1 1:100 Abcam, Cambridge, UK
  1. aBNIP3, BCL2/adenovirus E1B 19-kDa interacting protein 3; CAIX, carbonic anhydrase IX; ER, estrogen receptor; Glut-1, glucose transporter 1; LC3A, microtubule-associated protein 1 light chain 3α; LC3B, microtubule-associated protein 1 light chain 3β; MCT4, monocarboxylate transporter 4; PR, progesterone receptor.