Metabolic interaction between cancer cells and stromal cells according to breast cancer molecular subtype

Breast Cancer Research

Table 2 Source, clone and dilution of antibodies used in this study ^a

Antibody	Clone	Dilution	Company
Molecular subtype-related
ER	SP1	1:100	Thermo Scientific, Waltham, MA, USA
PR	PgR	1:50	Dako Denmark AS, Glostrup, Denmark
HER2	Polyclonal	1:1,500	Dako Denmark AS, Glostrup, Denmark
Ki-67	MIB-1	1:150	Dako Denmark AS, Glostrup, Denmark
Glycolysis-related
Glut-1	SPM498	1:200	Abcam, Cambridge, UK
CAIX	Polyclonal	1:100	Abcam, Cambridge, UK
MCT4	Polyclonal	1:100	Santa Cruz Biotechnology, Santa Cruz, CA, USA
Mitochondrial status-related
BNIP3	Ana40	1:100	Abcam, Cambridge, UK
Mitochondrial metabolism-related
ATP synthase	15H4C4	1:100	Abcam, Cambridge, UK
Glutaminase	Polyclonal	1:100	Abcam, Cambridge, UK
Autophagy-related
Beclin-1	Polyclonal	1:100	Abcam, Cambridge, UK
LC3A	EP1528Y	1:100	Abcam, Cambridge, UK
LC3B	Polyclonal	1:100	Abcam, Cambridge, UK
p62	SQSTM1	1:100	Abcam, Cambridge, UK

^aBNIP3, BCL2/adenovirus E1B 19-kDa interacting protein 3; CAIX, carbonic anhydrase IX; ER, estrogen receptor; Glut-1, glucose transporter 1; LC3A, microtubule-associated protein 1 light chain 3α; LC3B, microtubule-associated protein 1 light chain 3β; MCT4, monocarboxylate transporter 4; PR, progesterone receptor.

ISSN: 1465-542X