Phosphorylated-Akt is up-regulated in clinical DCIS specimens. (A) IHC of p-Akt in human DCIS specimens. Formalin-fixed, paraffin-embedded DCIS sections from 24 patients were stained with p-Akt. Phosphorylated-Akt intensity score: 0 = none, 1 = light, 2 = moderate, 3 = heavy. Bar = 100 μm. (B) Intensity and percentage expression pattern for p-Akt in human DCIS specimens. Phosho-Akt percentage score: 0 = <10%, 1 = 10% to 25%, 2 = 25% to 50%, 3 = >50%. (C) Up-regulation of p-Akt is associated with recurrent disease in human DCIS. DCIS, ductal carcinoma in situ; IHC, immunohistochemistry.