Table 4 Comparison between CK-19 RT-qPCR, and multiplex RT-qPCR for CK-19 detection
Molecular assay | CK-19RT-qPCR | |||
|---|---|---|---|---|
CK-19 multiplexRT-qPCR | Early breast cancer, (number = 254) | Verified metastasis, (number = 51) | ||
| Â | Positive | Negative | Positive | Negative |
Positive | 15 | 10 | 18 | 0 |
Negative | 21 | 208 | 3 | 30 |
Total (%) | 36 (14.2) | 218 (85.8) | 21 (41.2) | 30 (58.8) |
Kappa test | Moderate agreement: Kappa = 0.425 (P = 0.000) Pa = 0.000P = = Pp(P = 0.000) | Almost perfect agreement: Kappa = 0.876 (P = 0.000) | ||
Concordance (%) | 223 (87.8), Pa = 0.000 | 48 (94.1), Pa = 0.000 | ||