Figure 3
4T1 and 67NR tumors retain prolonged sensitivity to treatment with the dovitinib + NVP-BEZ235 combination. (A) Groups of 67NR tumor-bearing mice (n = 5) were treated daily with vehicle (PEG300), or with a combination of dovitinib (TKI, 20 mg/kg) and NVP-BEZ235 (10 mg/kg) for 7 days. Treatment was stopped for 5 days then resumed for 6 days and tumor growth was monitored. (B) Groups of 4T1 tumor-bearing mice (n = 6) were treated daily with vehicle (PEG300) or the combination of dovitinib (TKI, 20 mg/kg) + NVP-BEZ235 (10 mg/kg). After 10 days treatment, residual tumors were enzymatically digested, hematopoietic cell-depleted and 0.5 × 106 were re-injected into naïve mice (scheme in top panel). Starting 7 days after injection, groups of mice (n = 5) were treated daily for 11 days with (PEG300), dovitinib (TKI, 20 mg/kg), NVP-BEZ235 (10 mg/kg) or a combination of both, and tumor growth was monitored (lower portion of B). *P < 0.05 (Mann-Whitney U-test).