Figure 2

Regulation of estrogen receptor alpha expression by RhoB in MCF-7 cells and in vivo. (A) Cells were transfected with siControl (siC), siB1 or siB2 (left panel) or transduced with adenoviral vectors (multiplicity of infection (MOI) 300:1) (right panel) during 48 hours. (B) Mouse embryonic fibroblasts (MEFs) of RhoB-deficient mice were lysed. (C) MCF-7 cells were transfected with siC or siB2. Estrogen receptor alpha (ERα) phosphorylation was analyzed 48 hours later. (D) Cells were transduced with adenoviral vectors (MOI 300:1) during 48 hours, and 3 days later were treated by ICI-182, 780 or ethanol during 16 hours. (A) to (D) Protein expression was analyzed. Representative of three independent experiments. AdC, adenoviral control empty vector.