Figure 6From: ABCC5 supports osteoclast formation and promotes breast cancer metastasis to boneLoss of ABCC5 in breast cancer cells results in diminished osteoclast differentiation in vitro. Mouse bone marrow cells were primed with M-CSF and RANKL for 3 days and then cultured for 2 days untreated (negative control, NC), RANKL treated (positive control, PC), or conditioned media from 4T1-derived populations (scrambled shRNA (Scr), abcc5 shRNA 1, and abcc5 shRNA 2). Osteoclasts were counted for each condition, and the results are expressed as a percentage of the osteoclast number in the positive control. The data are presented as averages of five independent experiments (*P < 0.05). Representative images are presented for each group. The scale bar in the first image represents 100 μm and applies to all images.Back to article page