Generation of Menacalc scores and assessment of tumor heterogeneity in two independent breast cancer cohorts. A. Schematic representation of Mena, alternatively spliced inclusion of exons for Mena isoforms and binding sites for pan-Mena and Mena11a antibodies. B. Equation for the calculation of Menacalc scores and equations used to calculate Z scores for pan-Mena and Mena11a. × is the AQUA score for pan-Mena, Y is the AQUA score for Mena11a, μ is the mean of the AQUA scores for pan-Mena or Mena11a in each independent cohort and σ is standard deviation for pan-Mena or Mena11a AQUA scores for each independent cohort. C, D. Tumor heterogeneity of Menacalc in two independent cores of 501 individual patients from Cohort 1 (C) and 296 patients from Cohort 2 (D).