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Table 2 mRNA quantification by RT-qPCR of genes involved in breast cancer cell proliferation within wild type MCF7 and MCF7 cells stably transfected with 17β-HSD1 (MCF7-17βHSD1) and comparison with two-dimensional gel data.

From: 17beta-hydroxysteroid dehydrogenase type 1 modulates breast cancer protein profile and impacts cell migration

Descriptiona MCF7 MCF7-17βHSD1 Fold
regulationb
Correlation
2-D gel and
RT-qPCR
RT-qPCR value (mRNA copies/µg total RNA)   
Proliferating cell nuclear antigen (PCNA) 1,599,813 4,483,982 + 2.8 Yesc
Peroxiredoxin 2 4,078,760 8,585,424 + 2.1 Noc
Metastasis inhibition factor nm23 (nm23-H1) 5,366,763 19,356,416 + 3.6 Yesc
S-phase kinase-associated protein 1 (SKP1) 3,810,452 5,714,509 + 1.5 Yes
BRCA2 and CDKN1A interacting protein (BCCIP) 345,839 1,074,647 + 3.1 Noc
Ribonuclease/angiogenin inhibitor 1 (RNH1) 1,015,193 727,425 - 1.4 Yesc
  1. Standard deviations were < 10% of duplicates.
  2. aProteins were selected for reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) after their identification by mass spectrometry analysis of two-dimensional (2-D) gel protein spots.
  3. bFold regulation of mRNA levels in MCF7 cells stably transfected with 17β-HSD1 (MCF7-17βHSD1) compared to wild type MCF7 cells; +, fold increase; -, fold decrease).
  4. cMass spectrometry analysis showed that the two-dimensional spot contained several proteins including the indicated protein.