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Table 2 mRNA quantification by RT-qPCR of genes involved in breast cancer cell proliferation within wild type MCF7 and MCF7 cells stably transfected with 17β-HSD1 (MCF7-17βHSD1) and comparison with two-dimensional gel data.

From: 17beta-hydroxysteroid dehydrogenase type 1 modulates breast cancer protein profile and impacts cell migration

Descriptiona

MCF7

MCF7-17βHSD1

Fold

regulationb

Correlation

2-D gel and

RT-qPCR

RT-qPCR value (mRNA copies/µg total RNA)

  

Proliferating cell nuclear antigen (PCNA)

1,599,813

4,483,982

+ 2.8

Yesc

Peroxiredoxin 2

4,078,760

8,585,424

+ 2.1

Noc

Metastasis inhibition factor nm23 (nm23-H1)

5,366,763

19,356,416

+ 3.6

Yesc

S-phase kinase-associated protein 1 (SKP1)

3,810,452

5,714,509

+ 1.5

Yes

BRCA2 and CDKN1A interacting protein (BCCIP)

345,839

1,074,647

+ 3.1

Noc

Ribonuclease/angiogenin inhibitor 1 (RNH1)

1,015,193

727,425

- 1.4

Yesc

  1. Standard deviations were < 10% of duplicates.
  2. aProteins were selected for reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) after their identification by mass spectrometry analysis of two-dimensional (2-D) gel protein spots.
  3. bFold regulation of mRNA levels in MCF7 cells stably transfected with 17β-HSD1 (MCF7-17βHSD1) compared to wild type MCF7 cells; +, fold increase; -, fold decrease).
  4. cMass spectrometry analysis showed that the two-dimensional spot contained several proteins including the indicated protein.
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Breast Cancer Research

ISSN: 1465-542X

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