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Figure 2 | Breast Cancer Research

Figure 2

From: Loss of plakoglobin promotes decreased cell-cell contact, increased invasion, and breast cancer cell dissemination in vivo

Figure 2

Relative expression of plakoglobin and e-cadherin compared with GAPDH ± SEM before and after siRNA knockdown. (A) Scramble sequence or miRNA cassette 2 in MCF7 cells; (B) scramble sequence or miRNA cassette 3 in MCF7 cells; and (C) scramble sequence or miRNA cassette 2 in T47D cells. (D) Western blots showing γ-catenin and e-cadherin expression after transfection with scramble sequence or miRNA cassettes 2 and 3. (E) Immunohistochemical staining for γ-catenin, e-cadherin, and β-catenin (green). In the control cells, γ-catenin, e-cadherin, and β-catenin are expressed on the cell surface clearly demarcating the cell-cell junctions. In the knockdown lines, γ-catenin staining is reduced, and e-cadherin and β-catenin are detected in the nucleus and the cytoplasm.

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Breast Cancer Research

ISSN: 1465-542X

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