Relative expression of
± SEM before and after siRNA knockdown. (A) Scramble sequence or miRNA cassette 2 in MCF7 cells; (B) scramble sequence or miRNA cassette 3 in MCF7 cells; and (C) scramble sequence or miRNA cassette 2 in T47D cells. (D) Western blots showing γ-catenin and e-cadherin expression after transfection with scramble sequence or miRNA cassettes 2 and 3. (E) Immunohistochemical staining for γ-catenin, e-cadherin, and β-catenin (green). In the control cells, γ-catenin, e-cadherin, and β-catenin are expressed on the cell surface clearly demarcating the cell-cell junctions. In the knockdown lines, γ-catenin staining is reduced, and e-cadherin and β-catenin are detected in the nucleus and the cytoplasm.