Figure 7
JMJD6 represses TGF-β2 expression. RT-qPCR assays of TGF-βs after JMJD6 siRNA-mediated knockdown are shown in (A) MCF-7 and (B) MDA-MB231. Consistently, TGF-β2 and TGF-β1 mRNA levels were upregulated on JMJD6 knockdown. (C) Immunoblots showed that the amount of secreted TGF-β2 protein in conditioned media was higher after JMJD6 siRNA-mediated knockdown in MCF-7 and MDA-MB231. (D) RT-qPCR assay showed that MCF7 J1 OE clones had a lower level of TGF-β1 and TGF-β2 transcripts than did the Vec cells. (E) Western blot showed a dramatic decrease in secreted TGF-β2 protein, in the MCF-7 J1 clones, as compared with the Vec cells. NS, a Ponceau-stained blot of the conditioned media to demonstrate protein loading. Student's t- test was performed by using scrambled siRNA versus JMJD6 siRNA and Vec cells versus MCF-7J1 clones. *P ≤ 0.05; **P ≤ 0.005.