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Table 1 Primer sequences used for real-time RT-PCR

From: Bone marrow-derived, alternatively activated macrophages enhance solid tumor growth and lung metastasis of mammary carcinoma cells in a Balb/C mouse orthotopic model

mRNA

Primer sequences

Reference

M-CSF

Forward: 5'-TTGGCTTGGGATGATTCTCAG-3'

[28]

 

Backward: 5'-GCCCTGGGTCTGTCAGTCTC-3'

 

IP-10

Forward: 5'-CCCACGTGTTGAGATCATTG-3'

[29]

 

Backward: 5'-GCTCTCTGCTGTCCATCCAT-3'

 

RANTES

Forward: 5'-CCCTCACCATCATCCTCACT-3 '

[30]

 

Backward: 5'-CCACTTCTTCTCTGGGTTGG-3'

 

MCP-1

Forward: 5'-CAGCCAGATGCAGTTAACGC-3'

 
 

Backward: 5'-CCTCTCTCTTGAGCTTGGTG-3'

 

G-CSF

Forward: 5'-CATGGCTCAACTTTCTGCCCA-3'

[31]

 

Backward: 5'-TAGGTGGCACACAACTGCTC-3'

 

IL-1α

Forward: 5'-AAGACAAGCCTGTGTTGCTGAAGG-3'

[32]

 

Backward: 5'-CCAGAAGAAAATGAGGTCGGTC-3'

 

KC

Forward: 5'-CAAGAACATCCAGAGCTTGAAGGT-3'

[33]

 

Backward: 5'-GTGGCTATGACTTCGGTTTGG-3'

 

MIP-1α

Forward: 5'-ACCATGACACTCTGCAACCA-3'

[34]

 

Backward: 5'-CCCAGGTCTCTTTGGAGTCA-3'

 
  1. G-CSF, granulocyte colony-stimulating factor; HIF-1α, hypoxia-inducible factor-1α; IP-10, IFNγ-induced protein-10; KC, keratinocyte chemoattractant; M-CSF, macrophage colony-stimulating factor; MCP-1, monocyte chemotactic protein-1; MIP-1α, macrophage inflammatory protein-1 alpha; RANTES, regulated upon activation, normal T-cell expressed and secreted.
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Breast Cancer Research

ISSN: 1465-542X

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