Figure 1

Crk pathway signature in breast cancer is associated with basal subtype and tumor grade. Heatmap of Crk signature in NKI breast cancer dataset. Bars on the left indicate if genes increased or decreased expression in the T47D-CrkII cell line compared to control. The patients are ordered by correlation of the expression of these 151 genes in the patients sample to the Crk signature. Patients were separated into two subgroups: high correlation (correlation > 0.17, red) and low correlation (correlation < 0.17, blue). On heatmap, blue indicates increased expression; yellow indicates decreased expression. The Crk signature was used to examine overall 20 year outcome; the red line indicates patients who exhibit the Crk gene signature, whereas the blue line represents patients whose tumors do not express the signature (A). TMA-1 was stained with Ki67, CrkI/II, CrkII or CrkL specific antibodies in high (Grade 3) and low (Grade 1) grade tumors. Images taken using ImageScope; scale bar represents 50 μm. TMA-1 was utilized to examine CrkI/II and Ki67 expression in relation to tumor grade. Errors bars represent standard error of the mean (SEM) (B). Statistically significant data is illustrated as (* = P < 0.05, ** = P < 0.01, *** = P < 0.001).