Figure 2

Differential gene expression analysis in normal breast tissue (controls and breast cancer patients) and normal blood samples. Box plots displaying differentially expressed genes in normal breast samples from healthy controls (n = 29), matched normal breast tissue from breast cancer patients (n = 144) and blood samples from healthy individuals (n = 20) analyzed by Illumina HT12 v3 arrays and real-time PCR. (A) Gene expression versus genotype correlations between the genes SPP1, PAX8, MUC16 and IGFBP5 and two SNPs associated with higher expression of BRCA2, rs206081 (haplotype 3) and rs4942448 (haplotype 4), and one SNP associated with lower expression of BRCA2, rs9567578 (haplotype 2); P-values correspond to ANOVA testing. (B) Gene expression versus expression haplotype correlations for the genes MUC16 and SPP1; P-values correspond to a two-sided Student's t-Test. ANOVA, analysis of variance; PCR, polymerase chain reaction; SNP, single nucleotide polymorphism.