Figure 4

In vivo fluorescence imaging studies with post-image processing using the spectral separation. (A) In vivo and ex vivo fluorescence images of mice bearing 3T3/HER2 (left breast) or MDA-MB-468 (right breast) tumors as depicted by the Maestro imager, which employs an incremental filter and unmixing algorithms. The Alexa680 spectrum images shows a target specific image with Pan-Alexa680(SQ). In contrast, high background signal was observed for Pan-Alexa680(ON). Tra-ICG clearly visualized HER2 expressing 3T3/HER2 tumors in both groups. (B, C) The tumor to non-targeted tumor, neck and liver ratios for Alexa680 (B) or ICG (C) fluorescence intensity are depicted. Data are represented as mean ± s.d. (n = 4 mice).