Figure 4
From: Functional characterization of the 19q12 amplicon in grade III breast cancers
PLEKHF1, POP4, and CCNE1 are overexpressed when amplified at the protein level. (A) Western blot analysis of cell line panel with antibodies against POP4, PLEKHF1, CCNE1 and TSHZ3 in the breast cancer cell lines studied. In red, breast cancer cell lines harbouring amplification of the 19q12 locus. (B) Bar plots depicting POP4, PLEKHF1, CCNE1 and TSHZ3 protein expression quantified relative to the β-tubulin loading control, using ImageJ 1.44p (NIH, USA).