Figure 6

SNP-related variation in T-cell leukemia 1A expression and NF-κB activity in lymphoblastoid cell lines. SNP-related variation in T-cell leukemia 1A (TCL1A) expression and NF-κB transcriptional activity in three lymphoblastoid cell lines (LCLs) with variant (V) genotypes and three LCLs with wild type (WT) genotypes for the chromosome-14 SNPs after exposure to increasing concentrations of estradiol (E2), or E2 plus the estrogen receptor (ER) antagonist ICI-182,780. (A) Quantitative RT-PCR of TCL1A expression after exposure to increasing concentrations of E2 for 24 hours, followed by 0.01 nM E2 plus increasing concentrations of ICI-182,780 for an additional 24 hours. (B) The same six LCLs were transfected with an NF-κB reporter construct. After exposure to increasing concentrations of E2 for 24 hours, followed by 0.01 nM E2 plus increasing concentrations of ICI-182,780 for an additional 24 hours, luciferase activities were determined. All values represent the mean ± standard error of the mean of triplicate determinations.