T-cell leukemia 1A knockdown and overexpression and IL-17RA and IL-17 expression. (A) Relative T-cell leukemia 1A (TCL1A), IL-17RA and IL-17 mRNA expression levels in U2OS-estrogen receptor alpha (ERα) cells after TCL1A was knocked down using four different specific siRNAs. (B) Western blots of TCL1A, IL-17RA, IL-17 and β-actin, with or without TCL1A knockdown in U2OS-ERα cells. (C) Relative TCL1A, IL-17RA and IL-17 mRNA expression levels after TCL1A overexpression (OE) in U2OS-ERα cells. (D) Western blots of TCL1A, IL-17RA, IL-17 and β-actin, with or without TCL1A overexpression in U2OS-ERα cells. All data are the mean ± standard error of the mean of triplicate determinations.